Basic Information

Symbol
piR-hsa-3
RNA class
piRNA
Alias
hsa_piR_023378 hsa-piR-32054
Location (GRCh38)
Forensic tag(s)
Tissue/body fluid identification

Sequence & Structure

Transcript
piR-hsa-3
Sequence length
19 nt
GC content
0.5789

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-1.20 kcal/mol
Thermodynamic ensemble
Free energy: -2.28 kcal/mol
Frequency: 0.1722
Diversity: 4.25
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
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Thermodynamic Ensemble Prediction
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Forensic Context

A study in humans demonstrated that the piR-hsa-3 is a piwi-interacting RNA with potential for distinguishing forensically relevant biological fluids [Wang et al. DOI:10.1016/j.fsigen.2019.07.015]. The literature review further notes that piRNAs, including the piR-hsa-3, have been explored for body fluid identification, highlighting their forensic application [Li et al. DOI:10.1093/fsr/owad003]. A study in humans using massively parallel sequencing identified the piR-hsa-3 as an absolute specific marker (ASM) for semen, demonstrating its high specificity and expression in this body fluid [Liu et al. DOI:10.3390/genes13091530].