A study in humans demonstrated that the piR-hsa-3 is a piwi-interacting RNA with potential for distinguishing forensically relevant biological fluids [Wang et al. DOI:10.1016/j.fsigen.2019.07.015]. The literature review further notes that piRNAs, including the piR-hsa-3, have been explored for body fluid identification, highlighting their forensic application [Li et al. DOI:10.1093/fsr/owad003]. A study in humans using massively parallel sequencing identified the piR-hsa-3 as an absolute specific marker (ASM) for semen, demonstrating its high specificity and expression in this body fluid [Liu et al. DOI:10.3390/genes13091530].