Basic Information

Symbol
SNORA66
RNA Class
snoRNA
Alias
Small Nucleolar RNA, H/ACA Box 66 U66 RNA, U66 Small Nucleolar RNU66 SnoDB0063 SNORA66
Location (GRCh38)
1:92840719-92840851
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl RNAcentral

Sequence & Structure

Transcript
SNORA66
Sequence length
133 nt
GC content
0.4737

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-45.00 kcal/mol
Thermodynamic ensemble
Free energy: -47.82 kcal/mol
Frequency: 0.0102
Diversity: 8.89
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
.((((((((...((.((.(((((((........)))).)))..)).))....))))))))......((((((.(((((((((.(.....(((((((......)))))))....)))))))))..).)))))).
Thermodynamic Ensemble Prediction
.((((((((.{.((.((.(((((((........)))),,))..}).))....))))))))......((((((.{((((((((.(.....(((((((......)))))))....}))))))))..}.)))))).
Summary

No relevant information is available at the moment.

Forensic Context

A study in human backspatter from firearms demonstrated that the SNORA66 was successfully detected and selected as the best-suited reference gene from a panel of nine candidates for normalizing miRNA expression data from blood in these samples [Grabmüller et al. DOI:10.1007/s12024-015-9695-3]. A separate evaluation of candidate reference genes for miRNA analysis in forensically relevant body fluids found that the SNORA66 was top-ranked in pooled sample analysis but showed large inter-individual differences, leading to a lower rank in individual sample analysis [Sauer et al. DOI:10.1016/j.fsigen.2014.08.005]. A review of the literature indicates that in animals, including humans and mice, small nucleolar RNAs (snoRNAs) are differentially expressed in the brain and many are further processed into small RNAs with miRNA-like functions, guiding RNA-modifying enzymes for 2'-O-methylation and pseudouridylation [Mattick DOI:10.1002/bies.201000028].